Built 1827-29 by architect James Dempster of Greenock on the site of St Michael’s church which was founded in the late 13th century. Built with locally sourced red sandstone in a Tudor Gothic style with a three-bay front. The door to the southeast is adorned with grotesque ‘headstops’. There is a tiled vestibule in the three-stage tower and a curved stone staircase leading up the 90 foot tower.

In 1956-7, the interior was substantially reconstructed by the architectural firm Hay & Steel which witnessed the removal of its Georgian side galleries, leaving only an oak panelled rear gallery. The pipe organ is a Henry ‘Father’ Willis two manual organ of 1888, rebuilt in 1980 on its relocation to Mauchline from Strathbungo Parish Church in Glasgow. The stained glass, designed by the workshop of Powell & Sons,  in the southwest window dates from 1903 and depicts the biblical account of the healing of the Centurion’s Son.

The church at Mauchline was a centre of Covenanting feeling in the 17th century. Its then minister, the Rev George Young, signed both the National Covenant in Greyfriars’ Churchyard in 1638 and the Solemn League and Covenant in 1643. One of the kirk’s most important items on display is an original Covenanting battle flag, that was first used in a skirmish between the Covenanters and Royalists on nearby Mauchline Muir in 1648 and then carried into battle again on two further occasions, in 1679 at Drumclog and Bothwell Brig.

There are many interesting stones in the adjoining graveyard, most notably many memorials to contemporaries of Scotland’s national bard Robert Burns, who lived in Mauchline from 1784-86, and to Covenanter, James Smith.